Laboratory (Agriculture and Forestry)

Laboratory


Department of Agriculture and Forestry is well equipped with state of art labs to train students to advance their theoretical knowledge and sound practices of the profession.

The following are the labs run by the department under B.Sc. Agriculture:

Agriculture Meteorology

Agrometeorology is the study of weather and use of weather and climate information to enhance or expand agricultural crops and/or to increase crop production. Agrometeorology mainly involves the interaction of meteorological and hydrological factors, on one hand and agriculture, which encompasses horticulture, animal husbandry, and forestry.


Elementary Agriculture

Agricultural education can be taught at the elementary level, middle school level, secondary, post secondary and adult levels. Elementary agriculture is taught in public schools and private schools, and deals with such subjects as how plants and animals grow and how soil is farmed and conserved.

AGRONOMY LAB

This lab is well equipped with instruments that are used in agriculture field at the time of field management and cultivation. Also the lab contains various plant growth hormones and chemicals that can help enhance the growth and also facilitate various research activities.


S. NO. Name of the Experiment Objective
01 Effect of seed depth on crop yield To check effect of depth of seed on crop yield
02 Effect of nitrogen concentration on crop growth and yield 30 %
03 Study of various methods of seed sowing To study different ways of seed sowing
04 Study of various methods of seed sowing To check the effect of various plant growth hormones on and growth and yield of crops
05 Effect of bio fertilizers on crop yield and growth To check the effect of bio based fertilizers on yield and growth of the crops
06 Effect of tillage on crop growth and yield To check the effect of tillage activity on crop growth and yield
07 To study the different types of irrigation methods To check the irrigation methods

MICROBIOLOGY LAB

We have a well-equipped laboratory of microbiology containing Scanning Electron Microscope, Autoclave, Laminar Air Flow, Bacterial Identification Kit etc.


S. NO. Name of the Experiment Objective
01 Isolation of rhizobium from root nodule of leguminous plants Isolation of rhizobium from root nodule
02 Preparation of Nutrient Agar Media (NAM) Preparation of nutrient agar media with proper sterilization
03 Isolation of soil bacterium from soil sample Taking a soil sample and isolating soil bacterium from it
04 Cultivation of rhizobium in in-vitro condition Cultivate rhizobium in properly controlled and sterilized laboratory conditions
05 Differentiate between bacteria by gram staining method Differentiate between gram positive and gram negative bacteria by gram staining method
06 Isolation and identification of fungi from the infected plant part Identify the fungi from infected part of the plant
07 Effect of Pseudomonas aeuroginosa leguminous plants To check the growth of leguminous plants

GENETICS AND PLANT BREEDING LAB

We have a very well maintained laboratory for genetics experiments like protoplast isolation, hybridization etc.


S. NO. Name of the Experiment Objective
01 Isolating plant cell DNA Plant DNA isolation with the help of a centrifuge
02 Isolation of plant protoplast Protoplast isolation of plants by enzymatic method
03 To maintain the osmotic pressure after protoplast isolation Maintain the osmolarity of protoplasts by keeping it in a appropriate osmoticum
04 Gene delivery through Polyethylene glycol mediated gene transfer method Polyethylene glycol mediated
05 Study of various bacterial plasmids To study the bacterial plasmids
06 Statistical analysis of the various generations of plant To statistically analyse the various plant generations
07 Gene transfer by electroporation method To perform the electroporation method of gene transfer

SEED PRODUCTION FEED

We have a large incubator for germination of seeds in-vitro condition. All the high quality seeds which are rare are given various treatments inside the lab for ensuring its proper growth.


S. NO. Name of the Experiment Objective
01 Hardening of seeds through various chemicals Use of chemicals in hardening of seeds
02 To study the effect of chemicals on dormancy of seeds Chemicals effect on dormancy of seeds
03 To study various phases of seeds it undergo during plantlet production Various phase transition a seed undergoes during maturation
04 In-vitro Hybrid seeds preparation Conduct the experiment of hybrid seed preparation
05 Self-incompatibility of seeds To check the incompatibility of seeds
06 Seed hydration To check the hydration content of the seed
07 In-vitro albuminous seeds Preparation of albuninous seeds

BIOCHEMISTRY LAB

We have a well-equipped lab for performing biochemistry experiments such as testing of various biomolecules (carbohydrates, lipids, proteins and DNA). We have a refrigerated centrifuge, testing kits, plenty of glassware etc.


S. NO. Name of the Experiment Objective
01 To prepare buffer solution of a given normality Prepare buffer solution of a given normality
02 To determine pH of a given sample Determination of pH of a given sample
03 To study acid base titrations Acid base titration studies
04 To detect the presence of carbohydrate in a sample by molisch test Molisch tests for carbohydrates
05 To determine the presence of proteins in a given sample by ninhydrin test Detection of proteins by molisch test
06 To determine acid value of mustard oil Acid value of mustard oil
07 To determine peroxide value of mustard oil Peroxide value of mustard oil

SOIL TESTING LAB

In soil testing lab we have pycnometer for soil bulk density testing, digital pH meter for checking the soil’s pH, hot air oven for testing moisture content of soil and soil testing kit.


S. NO. Name of the Experiment Objective
01 To study various specimens of insects kept in the laboratory Study of various insect specimens of laboratory
02 Collection of insects from the outer environment Collection of insects from the campus
03 Study of the various insects collected Detailed study of the insects collected on the basis of their morphology
04 Learn the method of dissection of the insects Dissection of insects
05 Study of pest To study the morphology of pest
06 Study of different organs of insects To keenly study the different organs of insects

PLANT PATHOLOGY LAB

We have a very well equipped plant pathology lab in which we study different types of plant diseases. We have a high quality microscope for detection of microorganisms which attacks plants.


S. NO. Name of the Experiment Objective
01 Isolation of Albugo candida from crucifer plants Albugo candida isolation from crucifer plants
02 Identification of wheat disease in agriculture fields Wheat disease identification in agriculture fields
03 Analysis of wheat grain from diseased plants Wheat grain disease analysis from diseased plant
04 Study the disease cycle of Phytopthoreinfestans Study of Phytopthoreinfestans
05 Isolation of fungi Colletotrichumfalcicatumfrom sugarcane Colletotrichumfalcicatumisolation from sugarcane

TISSUE CULTURE LAB

Tissue culture lab of RC is very well maintained and has equipments such as Laminar air flow (LAF), Autoclave, culture room for growth of plants, distillation unit, and high quality chemicals and plant growth hormones (BAP, NAA, 24-D, zeatin etc.) and media (MS media, Gamborg’s media, etc.).


S. NO. Name of the Experiment Objective
01 Preparation of MS media Preparation of MS media for plant growth
02 Preparation of Gamborg’s or B5 media Preparation of Gamborg’s media for plant growth
03 Tissue culture of bamboos species by somatic organogenesis Somatic organogenesis of bamboos species (Bambu satulda)
04 Tissue culture and acclimatization of plants Tissue culture and acclimatization of plants
05 Calls induction of mango leaves Induction of callus from mango leaves
06 Micropropagation studies of Whithania somnifera To study the micropropagation of Whithania somnifera in-vitro
06 Isolation of DNA from plant cells To isolate the DNA from plant cells

POLYHOUSE

We have a very huge polyhouse with proper irrigation facilities for growing crops that are grown in different seasons. Polyhouse provides optimal temperature to various crops. All the treated crops are first grown inside the polyhouse and then transplanted in the outside field.


S. NO. Name of the Experiment Objective
01 Intercropping practice Applying intercropping method of cultivation in a polyhouse
02 Nursery preparation Prepare a nursery for lettuce and then transplanting it outside
03 Effect of water timing on growth and yield of crops To check the effect of water spraying time on growth and yield and plants
04 Excessive salt tolerance of plant To check the effect of excessive salt concentration on the growth and yield of the crops. Also identify which crop has the highest salt tolerance.
05 Cold shock to plants To give the crops excessive cold shock and check the behaviour of crops under that condition. Also identify which crop has the highest cold shock tolerance
06 Heat shock to plants To give the crops excessive heat shock and check the behaviour of crops under that condition. Also identify which crop has the highest heat shock tolerance